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發(fā)布時(shí)間：2025-07-29 13:24:30 更新時(shí)間：2025-07-28 13:24:30

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作者：中科光析科學(xué)技術(shù)研究所檢測(cè)中心

Introduction to Nitrogen Detection

Nitrogen detection, commonly abbreviated as "N detection," plays a pivotal role in modern environmental science, agriculture, and industrial processes, owing to nitrogen's fundamental importance as a key element in biological systems. Nitrogen is essential for life, forming the backbone of proteins, nucleic acids, and chlorophyll, yet its overabundance can lead to severe ecological imbalances such as eutrophication in water bodies, air pollution from nitrogen oxides, and soil degradation. Consequently, accurate nitrogen detection is critical for monitoring water quality, optimizing fertilizer use in farming, ensuring food safety, and complying with environmental regulations. The significance of N detection extends to various sectors; for instance, in wastewater treatment plants, it helps prevent harmful algal blooms, while in agricultural settings, it aids in precise nutrient management to boost crop yields sustainably. As global concerns over pollution and resource conservation rise, advancements in detection technologies have evolved to provide rapid, sensitive, and cost-effective solutions, making it an indispensable tool in contemporary analytical chemistry and sustainability initiatives.

檢測(cè)項(xiàng)目 (Detection Projects)

Nitrogen detection projects encompass a range of specific analytes tailored to different applications. Key projects include total nitrogen (TN) measurement, which assesses the sum of all nitrogen forms in a sample, such as organic nitrogen, ammonia, and nitrates, often used in comprehensive environmental monitoring. Ammonia nitrogen (NH3-N) detection focuses on quantifying ammonia levels, crucial for evaluating wastewater discharge impacts and aquatic toxicity. Nitrate nitrogen (NO3-N) and nitrite nitrogen (NO2-N) projects are vital for assessing drinking water safety and agricultural runoff risks, as high concentrations can cause health issues like methemoglobinemia. Other specialized projects include Kjeldahl nitrogen determination for organic nitrogen in soils or food samples, and particulate nitrogen analysis in air quality studies. Each project targets distinct nitrogen compounds to address specific regulatory or operational needs, ensuring targeted interventions in pollution control and resource management.

檢測(cè)儀器 (Detection Instruments)

A variety of advanced instruments are employed for nitrogen detection, each designed for specific accuracy and efficiency. Spectrophotometers, such as UV-Vis models, are widely used for colorimetric methods, like the Nesslerization technique for ammonia nitrogen, offering high sensitivity through light absorption measurements. Chromatographic instruments, including ion chromatography (IC) systems, are ideal for separating and quantifying nitrate and nitrite ions in complex matrices like groundwater or food extracts. Elemental analyzers, such as the Kjeldahl apparatus, utilize digestion and titration to determine total nitrogen in organic samples, while combustion analyzers apply high-temperature oxidation to measure TN rapidly. Electrochemical sensors, like ammonia-selective electrodes, provide on-site real-time monitoring for agricultural or industrial applications. Lastly, advanced tools like inductively coupled plasma mass spectrometry (ICP-MS) offer ultra-trace detection for research purposes. These instruments are selected based on factors like sample type, required precision, and throughput, ensuring reliable data across diverse scenarios.

檢測(cè)方法 (Detection Methods)

Detection methods for nitrogen are standardized to ensure reproducibility and reliability. The Kjeldahl method is a classic approach for total nitrogen, involving sample digestion with sulfuric acid to convert nitrogen to ammonium sulfate, followed by distillation and titration to quantify results. Colorimetric methods, such as the phenol-hypochlorite reaction for ammonia nitrogen, use reagents to produce a colored complex measured via spectrophotometry. For nitrate and nitrite detection, the cadmium reduction method employs chemical reduction to nitrite, which is then diazotized and measured. Advanced techniques include gas chromatography-mass spectrometry (GC-MS) for volatile organic nitrogen compounds, and enzymatic assays that use specific enzymes like nitrate reductase for rapid field testing. Automation via flow injection analysis (FIA) enhances speed and minimizes human error. Each method requires careful sample preparation, such as filtration or preservation, to avoid interferences and ensure accuracy in diverse matrices like water, soil, or biological tissues.

檢測(cè)標(biāo)準(zhǔn) (Detection Standards)

Nitrogen detection adheres to stringent international and national standards to guarantee uniformity and compliance. Key international standards include ISO 5667-3 for water sampling procedures, which outlines protocols for collecting and handling samples to prevent contamination. The ISO 11905 series specifies methods for nitrogen determination in water, such as ISO 11905-1 for Kjeldahl nitrogen and ISO 11905-2 for nitrate and nitrite via ion chromatography. EPA methods, like EPA 350.1 for ammonia nitrogen and EPA 353.2 for nitrate-nitrite, are widely adopted in the U.S. for environmental monitoring. In China, the GB/T 5750 standard governs drinking water testing, with specific sections for nitrogen compounds, while GB 11894 provides guidelines for total nitrogen in wastewater. Additionally, industry-specific standards, such as AOAC International for food and agricultural samples, ensure safety and quality. These standards mandate calibration, quality control checks, and validation to uphold data integrity and support global environmental and health regulations.